Antioxidant activities and molecular mechanisms of the ethanol extracts of Baccharis propolis and Eucalyptus propolis in RAW64.7 cells.
Identifieur interne : 001A06 ( Main/Exploration ); précédent : 001A05; suivant : 001A07Antioxidant activities and molecular mechanisms of the ethanol extracts of Baccharis propolis and Eucalyptus propolis in RAW64.7 cells.
Auteurs : Jianglin Zhang [République populaire de Chine] ; Xiaoge Shen [République populaire de Chine] ; Kai Wang [République populaire de Chine] ; Xueping Cao [République populaire de Chine] ; Cuiping Zhang [République populaire de Chine] ; Huoqing Zheng [République populaire de Chine] ; Fuliang Hu [République populaire de Chine]Source :
- Pharmaceutical biology [ 1744-5116 ] ; 2016.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Antioxydants (isolement et purification), Antioxydants (pharmacologie), Antioxydants (toxicité), Baccharis (MeSH), Cellules RAW 264.7 (MeSH), Chromatographie en phase liquide à haute performance (MeSH), Espèces réactives de l'oxygène (métabolisme), Eucalyptus (MeSH), Extracellular Signal-Regulated MAP Kinases (métabolisme), Facteur-2 apparenté à NF-E2 (métabolisme), Macrophages (effets des médicaments et des substances chimiques), Macrophages (métabolisme), Phénols (isolement et purification), Phénols (pharmacologie), Phénols (toxicité), Propolis (composition chimique), Relation dose-effet des médicaments (MeSH), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques), Solvants (composition chimique), Souris (MeSH), Stress oxydatif (effets des médicaments et des substances chimiques), Stress oxydatif (génétique), Survie cellulaire (effets des médicaments et des substances chimiques), Transduction du signal (effets des médicaments et des substances chimiques), p38 Mitogen-Activated Protein Kinases (métabolisme), Éthanol (composition chimique).
- MESH :
- composition chimique : Propolis, Solvants, Éthanol.
- effets des médicaments et des substances chimiques : Macrophages, Régulation de l'expression des gènes, Stress oxydatif, Survie cellulaire, Transduction du signal.
- génétique : Stress oxydatif.
- isolement et purification : Antioxydants, Phénols.
- métabolisme : Espèces réactives de l'oxygène, Extracellular Signal-Regulated MAP Kinases, Facteur-2 apparenté à NF-E2, Macrophages, p38 Mitogen-Activated Protein Kinases.
- pharmacologie : Antioxydants, Phénols.
- toxicité : Antioxydants, Phénols.
- Animaux, Baccharis, Cellules RAW 264.7, Chromatographie en phase liquide à haute performance, Eucalyptus, Relation dose-effet des médicaments, Souris.
English descriptors
- KwdEn :
- Animals (MeSH), Antioxidants (isolation & purification), Antioxidants (pharmacology), Antioxidants (toxicity), Baccharis (MeSH), Cell Survival (drug effects), Chromatography, High Pressure Liquid (MeSH), Dose-Response Relationship, Drug (MeSH), Ethanol (chemistry), Eucalyptus (MeSH), Extracellular Signal-Regulated MAP Kinases (metabolism), Gene Expression Regulation (drug effects), Macrophages (drug effects), Macrophages (metabolism), Mice (MeSH), NF-E2-Related Factor 2 (metabolism), Oxidative Stress (drug effects), Oxidative Stress (genetics), Phenols (isolation & purification), Phenols (pharmacology), Phenols (toxicity), Propolis (chemistry), RAW 264.7 Cells (MeSH), Reactive Oxygen Species (metabolism), Signal Transduction (drug effects), Solvents (chemistry), p38 Mitogen-Activated Protein Kinases (metabolism).
- MESH :
- chemical , chemistry : Ethanol, Propolis, Solvents.
- chemical , isolation & purification : Antioxidants, Phenols.
- chemical , metabolism : Extracellular Signal-Regulated MAP Kinases, NF-E2-Related Factor 2, Reactive Oxygen Species, p38 Mitogen-Activated Protein Kinases.
- chemical , pharmacology : Antioxidants, Phenols.
- chemical , toxicity : Antioxidants, Phenols.
- drug effects : Cell Survival, Gene Expression Regulation, Macrophages, Oxidative Stress, Signal Transduction.
- genetics : Oxidative Stress.
- metabolism : Macrophages.
- Animals, Baccharis, Chromatography, High Pressure Liquid, Dose-Response Relationship, Drug, Eucalyptus, Mice, RAW 264.7 Cells.
Abstract
Context Numerous studies have reported that propolis possesses strong antioxidant activities. However, their antioxidant molecular mechanisms are unclear. Objective We utilize ethanol extracts of Chinese propolis (EECP) as a reference to compare ethanol extracts of Eucalyptus propolis (EEEP) with ethanol extracts of Baccharis propolis (EEBGP) based on their antioxidant capacities and underlying molecular mechanisms. Materials and methods HPLC and chemical analysis are utilized to evaluate compositions and antioxidant activities. ROS-eliminating effects of EEBGP (20-75 μg/mL), EEEP (1.25-3.75 μg/mL) and EECP (1.25-5 μg/mL) are also determined by flow cytometry analysis. Moreover, we compared antioxidant capacities by determining their effects on expressions of antioxidant genes in RAW264.7 cells with qRT-PCR, western blot and confocal microscopy analysis. Results EEBGP mainly contains chlorogenic acid (8.98 ± 0.86 mg/g), kaempferide (11.18 ± 8.31 mg/g) and artepillin C (107.70 ± 10.86 mg/g), but EEEP contains 10 compositions, whereas EECP contains 17 compositions. Meantime, although EEEP shows DPPH (IC50 19.55 ± 1.28), ABTS (IC50 20.0 ± 0.31) and reducing power (2.70 ± 0.08 mmol TE/g) better than EEBGP's DPPH (IC50 43.85 ± 0.54), ABTS (IC50 38.2 ± 0.33) and reducing power (1.53 ± 0.05 mmol TE/g), EEBGP exerts much higher ROS inhibition rate (40%) than EEEP (under 20%). Moreover, EEBGP strengthen antioxidant system by activating p38/p-p38 and Erk/p-Erk kinase via accelerating nucleus translocation of Nrf2. EEEP and EECP improve antioxidant gene expression only via Erk/p-Erk kinase-Nrf2 signalling pathway. Discussion and conclusion EEBGP and EEEP exert antioxidant activities via different molecular mechanisms, which may depend on chemical compositions.
DOI: 10.3109/13880209.2016.1151444
PubMed: 27049854
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Shen, Xiaoge" sort="Shen, Xiaoge" uniqKey="Shen X" first="Xiaoge" last="Shen">Xiaoge Shen</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Wang, Kai" sort="Wang, Kai" uniqKey="Wang K" first="Kai" last="Wang">Kai Wang</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Cao, Xueping" sort="Cao, Xueping" uniqKey="Cao X" first="Xueping" last="Cao">Xueping Cao</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Cuiping" sort="Zhang, Cuiping" uniqKey="Zhang C" first="Cuiping" last="Zhang">Cuiping Zhang</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Huoqing" sort="Zheng, Huoqing" uniqKey="Zheng H" first="Huoqing" last="Zheng">Huoqing Zheng</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author><author><name sortKey="Hu, Fuliang" sort="Hu, Fuliang" uniqKey="Hu F" first="Fuliang" last="Hu">Fuliang Hu</name><affiliation wicri:level="1"><nlm:affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>a College of Animal Science , Zhejiang University , Hangzhou </wicri:regionArea><wicri:noRegion>Hangzhou </wicri:noRegion></affiliation></author></analytic><series><title level="j">Pharmaceutical biology</title><idno type="eISSN">1744-5116</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Antioxidants (isolation & purification)</term><term>Antioxidants (pharmacology)</term><term>Antioxidants (toxicity)</term><term>Baccharis (MeSH)</term><term>Cell Survival (drug effects)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Dose-Response Relationship, Drug (MeSH)</term><term>Ethanol (chemistry)</term><term>Eucalyptus (MeSH)</term><term>Extracellular Signal-Regulated MAP Kinases (metabolism)</term><term>Gene Expression Regulation (drug effects)</term><term>Macrophages (drug effects)</term><term>Macrophages (metabolism)</term><term>Mice (MeSH)</term><term>NF-E2-Related Factor 2 (metabolism)</term><term>Oxidative Stress (drug effects)</term><term>Oxidative Stress (genetics)</term><term>Phenols (isolation & purification)</term><term>Phenols (pharmacology)</term><term>Phenols (toxicity)</term><term>Propolis (chemistry)</term><term>RAW 264.7 Cells (MeSH)</term><term>Reactive Oxygen Species (metabolism)</term><term>Signal Transduction (drug effects)</term><term>Solvents (chemistry)</term><term>p38 Mitogen-Activated Protein Kinases (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Antioxydants (isolement et purification)</term><term>Antioxydants (pharmacologie)</term><term>Antioxydants (toxicité)</term><term>Baccharis (MeSH)</term><term>Cellules RAW 264.7 (MeSH)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Eucalyptus (MeSH)</term><term>Extracellular Signal-Regulated MAP Kinases (métabolisme)</term><term>Facteur-2 apparenté à NF-E2 (métabolisme)</term><term>Macrophages (effets des médicaments et des substances chimiques)</term><term>Macrophages (métabolisme)</term><term>Phénols (isolement et purification)</term><term>Phénols (pharmacologie)</term><term>Phénols (toxicité)</term><term>Propolis (composition chimique)</term><term>Relation dose-effet des médicaments (MeSH)</term><term>Régulation de l'expression des gènes (effets des médicaments et des substances chimiques)</term><term>Solvants (composition chimique)</term><term>Souris (MeSH)</term><term>Stress oxydatif (effets des médicaments et des substances chimiques)</term><term>Stress oxydatif (génétique)</term><term>Survie cellulaire (effets des médicaments et des substances chimiques)</term><term>Transduction du signal (effets des médicaments et des substances chimiques)</term><term>p38 Mitogen-Activated Protein Kinases (métabolisme)</term><term>Éthanol (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ethanol</term><term>Propolis</term><term>Solvents</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antioxidants</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Extracellular Signal-Regulated MAP Kinases</term><term>NF-E2-Related Factor 2</term><term>Reactive Oxygen Species</term><term>p38 Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antioxidants</term><term>Phenols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Antioxidants</term><term>Phenols</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Propolis</term><term>Solvants</term><term>Éthanol</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Gene Expression Regulation</term><term>Macrophages</term><term>Oxidative Stress</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Macrophages</term><term>Régulation de l'expression des gènes</term><term>Stress oxydatif</term><term>Survie cellulaire</term><term>Transduction du signal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oxidative Stress</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Stress oxydatif</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antioxydants</term><term>Phénols</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Espèces réactives de l'oxygène</term><term>Extracellular Signal-Regulated MAP Kinases</term><term>Facteur-2 apparenté à NF-E2</term><term>Macrophages</term><term>p38 Mitogen-Activated Protein Kinases</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antioxydants</term><term>Phénols</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Antioxydants</term><term>Phénols</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Baccharis</term><term>Chromatography, High Pressure Liquid</term><term>Dose-Response Relationship, Drug</term><term>Eucalyptus</term><term>Mice</term><term>RAW 264.7 Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Baccharis</term><term>Cellules RAW 264.7</term><term>Chromatographie en phase liquide à haute performance</term><term>Eucalyptus</term><term>Relation dose-effet des médicaments</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Context Numerous studies have reported that propolis possesses strong antioxidant activities. However, their antioxidant molecular mechanisms are unclear. Objective We utilize ethanol extracts of Chinese propolis (EECP) as a reference to compare ethanol extracts of Eucalyptus propolis (EEEP) with ethanol extracts of Baccharis propolis (EEBGP) based on their antioxidant capacities and underlying molecular mechanisms. Materials and methods HPLC and chemical analysis are utilized to evaluate compositions and antioxidant activities. ROS-eliminating effects of EEBGP (20-75 μg/mL), EEEP (1.25-3.75 μg/mL) and EECP (1.25-5 μg/mL) are also determined by flow cytometry analysis. Moreover, we compared antioxidant capacities by determining their effects on expressions of antioxidant genes in RAW264.7 cells with qRT-PCR, western blot and confocal microscopy analysis. Results EEBGP mainly contains chlorogenic acid (8.98 ± 0.86 mg/g), kaempferide (11.18 ± 8.31 mg/g) and artepillin C (107.70 ± 10.86 mg/g), but EEEP contains 10 compositions, whereas EECP contains 17 compositions. Meantime, although EEEP shows DPPH (IC50 19.55 ± 1.28), ABTS (IC50 20.0 ± 0.31) and reducing power (2.70 ± 0.08 mmol TE/g) better than EEBGP's DPPH (IC50 43.85 ± 0.54), ABTS (IC50 38.2 ± 0.33) and reducing power (1.53 ± 0.05 mmol TE/g), EEBGP exerts much higher ROS inhibition rate (40%) than EEEP (under 20%). Moreover, EEBGP strengthen antioxidant system by activating p38/p-p38 and Erk/p-Erk kinase via accelerating nucleus translocation of Nrf2. EEEP and EECP improve antioxidant gene expression only via Erk/p-Erk kinase-Nrf2 signalling pathway. Discussion and conclusion EEBGP and EEEP exert antioxidant activities via different molecular mechanisms, which may depend on chemical compositions. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27049854</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>07</Day></DateCompleted><DateRevised><Year>2017</Year><Month>02</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1744-5116</ISSN><JournalIssue CitedMedium="Internet"><Volume>54</Volume><Issue>10</Issue><PubDate><Year>2016</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Pharmaceutical biology</Title><ISOAbbreviation>Pharm Biol</ISOAbbreviation></Journal><ArticleTitle>Antioxidant activities and molecular mechanisms of the ethanol extracts of Baccharis propolis and Eucalyptus propolis in RAW64.7 cells.</ArticleTitle><Pagination><MedlinePgn>2220-35</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3109/13880209.2016.1151444</ELocationID><Abstract><AbstractText>Context Numerous studies have reported that propolis possesses strong antioxidant activities. However, their antioxidant molecular mechanisms are unclear. Objective We utilize ethanol extracts of Chinese propolis (EECP) as a reference to compare ethanol extracts of Eucalyptus propolis (EEEP) with ethanol extracts of Baccharis propolis (EEBGP) based on their antioxidant capacities and underlying molecular mechanisms. Materials and methods HPLC and chemical analysis are utilized to evaluate compositions and antioxidant activities. ROS-eliminating effects of EEBGP (20-75 μg/mL), EEEP (1.25-3.75 μg/mL) and EECP (1.25-5 μg/mL) are also determined by flow cytometry analysis. Moreover, we compared antioxidant capacities by determining their effects on expressions of antioxidant genes in RAW264.7 cells with qRT-PCR, western blot and confocal microscopy analysis. Results EEBGP mainly contains chlorogenic acid (8.98 ± 0.86 mg/g), kaempferide (11.18 ± 8.31 mg/g) and artepillin C (107.70 ± 10.86 mg/g), but EEEP contains 10 compositions, whereas EECP contains 17 compositions. Meantime, although EEEP shows DPPH (IC50 19.55 ± 1.28), ABTS (IC50 20.0 ± 0.31) and reducing power (2.70 ± 0.08 mmol TE/g) better than EEBGP's DPPH (IC50 43.85 ± 0.54), ABTS (IC50 38.2 ± 0.33) and reducing power (1.53 ± 0.05 mmol TE/g), EEBGP exerts much higher ROS inhibition rate (40%) than EEEP (under 20%). Moreover, EEBGP strengthen antioxidant system by activating p38/p-p38 and Erk/p-Erk kinase via accelerating nucleus translocation of Nrf2. EEEP and EECP improve antioxidant gene expression only via Erk/p-Erk kinase-Nrf2 signalling pathway. Discussion and conclusion EEBGP and EEEP exert antioxidant activities via different molecular mechanisms, which may depend on chemical compositions. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jianglin</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Xiaoge</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Kai</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Xueping</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Cuiping</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Huoqing</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Fuliang</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>a College of Animal Science , Zhejiang University , Hangzhou , P.R. China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Pharm Biol</MedlineTA><NlmUniqueID>9812552</NlmUniqueID><ISSNLinking>1388-0209</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000975">Antioxidants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D051267">NF-E2-Related Factor 2</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C495636">Nfe2l2 protein, mouse</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010636">Phenols</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012997">Solvents</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9009-62-5</RegistryNumber><NameOfSubstance UI="D011429">Propolis</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D048049">Extracellular Signal-Regulated MAP Kinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.24</RegistryNumber><NameOfSubstance UI="D048051">p38 Mitogen-Activated Protein Kinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000975" MajorTopicYN="N">Antioxidants</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031175" MajorTopicYN="Y">Baccharis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005052" MajorTopicYN="Y">Eucalyptus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D048049" MajorTopicYN="N">Extracellular Signal-Regulated MAP Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051267" MajorTopicYN="N">NF-E2-Related Factor 2</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010636" MajorTopicYN="N">Phenols</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011429" MajorTopicYN="N">Propolis</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000067996" MajorTopicYN="N">RAW 264.7 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012997" MajorTopicYN="N">Solvents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D048051" MajorTopicYN="N">p38 Mitogen-Activated Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">MAPKs</Keyword><Keyword MajorTopicYN="N">Nrf2</Keyword><Keyword MajorTopicYN="N">poplar propolis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27049854</ArticleId><ArticleId IdType="doi">10.3109/13880209.2016.1151444</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Jianglin" sort="Zhang, Jianglin" uniqKey="Zhang J" first="Jianglin" last="Zhang">Jianglin Zhang</name></noRegion><name sortKey="Cao, Xueping" sort="Cao, Xueping" uniqKey="Cao X" first="Xueping" last="Cao">Xueping Cao</name><name sortKey="Hu, Fuliang" sort="Hu, Fuliang" uniqKey="Hu F" first="Fuliang" last="Hu">Fuliang Hu</name><name sortKey="Shen, Xiaoge" sort="Shen, Xiaoge" uniqKey="Shen X" first="Xiaoge" last="Shen">Xiaoge Shen</name><name sortKey="Wang, Kai" sort="Wang, Kai" uniqKey="Wang K" first="Kai" last="Wang">Kai Wang</name><name sortKey="Zhang, Cuiping" sort="Zhang, Cuiping" uniqKey="Zhang C" first="Cuiping" last="Zhang">Cuiping Zhang</name><name sortKey="Zheng, Huoqing" sort="Zheng, Huoqing" uniqKey="Zheng H" first="Huoqing" last="Zheng">Huoqing Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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